Effect+of+temperature+and+pH+on+enzyme+action


 * __Investigating the effect of temperature and pH on enzyme action__**


 * __PART A__**

__Table 1: Results of the effect of temperature on enzyme action__ __Interpreting the results__
 * Test tube || Contents || Temperature of water bath || Iodine test || Conclusion ||
 * A1 || 5cm³ of starch solution + 3cm³ of amylase || 0°C || Solution turned blue black || Starch digestion has not occurred ||
 * B1 || 5cm³ of starch solution + 3cm³ of amylase || 37°C || Solution turned yellowish brown || Starch digestion has occurred ||
 * C1 || 5cm³ of starch solution + 3cm³ of amylase || 100°C || Solution turned blue black || Starch digestion has not occurred ||
 * D1 || 5cm³ of starch solution + 3cm³ of distilled water || 37°C || Solution turned blue black || Starch digestion has not occurred ||

Tube B1 shows that starch digestion has occurred. When performing the iodine test, the solution turned blue black which means that starch digestion has occurred.
 * //1. Which tube shows that starch digestion has occurred? Give your reasons//**//.//

Test tubes A1, C1 and D1.
 * //2. Which of the test-tubes contained mixtures that gave a blue-black colour?//**

//**3. Why did the mixtures in the tubes stated in (2) remain blue-black?**// Starch was present in the mixture therefore, they remained blue-black.

It serves as a control for the experiment.
 * //4.//** **//What purpose does the tube D1 serve?//**

//**5. Using your knowledge of the structure of starch, describe the test you would use to detect the presence of the substancee produced as a result of the reaction in the test tube stated in (1) above?**// When starch digestion occurs, glucose is produced. Therefore, Benedict's solution is a suitable method.

//**6. What conclusion(s), based on the observation in this experiment, can be made about the effect of temperature on enzyme action.**//

__**PART B**

Table 2: Results of starch digestion using amylase in different conditions__


 * Test tube || Contents || Iodine test || Conclusion ||
 * < W ||< 2cm³ of starch solution + 2cm³ of HCl + 2cm³ of amylase ||< Solution turned blue black ||< Starch digestion has not occurred ||
 * X || 2cm³ of starch solution + 2cm³ of distilled water+ 2cm³ of amylase || Solution turned yellowish brown || Starch digestion has occurred ||
 * Y || 2cm³ of starch solution + 2cm³ of NH4OH + 2cm³ of amylase || Solution turned blue black || Starch digestion has not occurred ||
 * Z || 2cm³ of starch solution + 4cm³ of distilled water || Solution turned blue black || Starch digestion has not occurred ||

__Interpreting the results__

//**1. Which tube shows that starch digestion has occurred? Give your reasons.**//


 * //2. Which of the test-tubes contained mixtures that gave a blue-black colour?//**


 * //3. Why did the mixtures in the tubes stated in (2) remain blue-black?//**


 * //4.//** //**What conclusion(s), based on the observation in this experiment, can be made about the effect of pH on enzyme action.**//


 * //5. How does the temperature and pH affect enzyme action?//**